Stem Cell Renewal and Cell-Cell Communication by Kursad Turksen

Author:Kursad Turksen
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

4 Notes

1.Matrigel™ should always be kept on ice during thawing in order to prevent gelling.

2.If not used immediately, the culture plate should be sealed to avoid evaporation of Matrigel™ working solution. Culture plates can be sealed with Parafilm® and can be stored at 4 °C for up to 1 week after coating. Prior to using stored Matrigel™ coated plates, allow the plates to equilibrate with room temperature for 15 min.

3.Aspirate Matrigel™ solution immediately prior to seeding of cells. Do not allow the Matrigel™ coated surfaces to dry out.

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